Validation of Suitable Reference Genes for Assessing Gene Expression of MicroRNAs in Lonicera japonica
نویسندگان
چکیده
منابع مشابه
Validation of Suitable Reference Genes for Assessing Gene Expression of MicroRNAs in Lonicera japonica
MicroRNAs (miRNAs), which play crucial regulatory roles in plant secondary metabolism and responses to the environment, could be developed as promising biomarkers for different varieties and production areas of herbal medicines. However, limited information is available for miRNAs from Lonicera japonica, which is widely used in East Asian countries owing to various pharmaceutically active secon...
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Reverse transcription-qPCR (RT-qPCR) has become a popular method for gene expression studies. Its results require data normalization by housekeeping genes. No single gene is proved to be stably expressed under all experimental conditions. Therefore, systematic evaluation of reference genes is necessary. With the aim to identify optimum reference genes for RT-qPCR analysis of gene expression in ...
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MiRNAs function in post-transcriptional regulation of gene expression and play very important roles in plant development. Lonicera japonica is one of the important medicinal plants in China. However, few studies on the discovery of conserved and novel miRNAs from L. japonica were reported. In this study, we employed deep sequencing technology to identify miRNAs in leaf and flower tissues of L. ...
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2016
ISSN: 1664-462X
DOI: 10.3389/fpls.2016.01101